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Objective:To examine the relaxant effects of hydro-ethanolic,macerated aqueous(MA) and lipidfree macerated aqueous(LFMA) extract of Tymus vulgaris on tracheal chains of guinea pigs.Methods:The relaxant effects of five cumulative concentrations of each extract(0.4,0.8,1.2,1.6 and 2.0 g/100 mL) were compared with saline as negative control and five cumulative concentrations of theophylline(0.1,0.2,0.4,0.6 and 0.8 mmol/L) on precontracted tracheal smooth muscle of guinea pig with 60 mmol/L KCI(group 1) and 10 μmol/L methacholine(group 2,n=6 for each group).Results:In group 1 all concentrations of theophylline,three higher concentrations of hydro-ethanolic,two concentrations of LFMA and last concentration of MA extracts showed significant relaxant effects compared with that of saline(P<0.05 or P<0.01).Two lower concentrations of LFMA and all concentrations of MA except higher one caused contraction compared with saline(P<0.05 or 0.01).In group 2 experiments,all concentrations of theophylline,hydro-ethanolic,MA and LFMA extracts showed significant relaxant effects compared to that of saline(P<0.05 or P<0.01).In both groups,the relaxant effect of all concentrations of hydro-ethanolic extract were significantly higher than most concentrations of others(P<0.05 or P<0.01).The relaxant effect of different concentrations of three extracts were significantly greater in group 2 compared with group 1 experiments(all P<0.01).There were significantly positive correlations between the relaxant effects and concentrations for theophylline and ail extracts in both groups(P<0.05 or P<0.01).Conclusion:Hydro-ethanolic extract has a potent weaker relaxant effect for other extracts from Tymus vulgaris on tracheal chains of guinea pigs.
Objective: To examine the relaxant effects of hydro-ethanolic, macerated aqueous (MA) and lipidfree macerated aqueous (LFMA) extract of Tymus vulgaris on tracheal chains of guinea pigs.Methods: The relaxant effects of five cumulative concentrations of each extract (0.4, 0.8, 1.2, 1.6 and 2.0 g / 100 mL) were compared with saline as negative control and five cumulative concentrations of theophylline (0.1, 0.2, 0.4, 0.6 and 0.8 mmol / L) on precontracted smooth muscle of guinea pig with 60 mmol / L KCI (group 1) and 10 μmol / L methacholine (group 2, n = 6 for each group). Results: In group 1 all concentrations of theophylline, three higher concentrations of hydro- ethanolic, two concentrations of LFMA and last concentration of MA extracts showed significant relaxant effects compared with that of saline (P <0.05 or P <0.01). Two sets of LFMA and all concentrations of MA except higher one were contracted with saline (P <0.05 or 0.01) .In group 2 experiments, all concentrations of theophyll ine, hydro-ethanolic, MA and LFMA extracts showed significant relaxant effects compared to that of saline (P <0.05 or P <0.01). In both groups, the relaxant effect of all concentrations of hydro- of all other factors (P <0.05 or P <0.01). The relaxant effect of different concentrations of three extracts were significantly greater in group 2 compared with group 1 experiments for theophylline and ail extracts in both groups (P <0.05 or P <0.01) .Conclusion: Hydro-ethanolic extract has a potent weaker relaxant effect for other extracts from Tymus vulgaris on tracheal chains of guinea pigs.