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研究神经系活动的关键是弄清神经元中何物质起调节神经递质及其受体的作用。DNA 重组技术为分子水平研究神经调节因子提供了保证。原位杂交方法为重要的分子学研究工具。降钙素基因相关的肽(CGRP)是37个氨基酸的多肽,为降钙素基因转录翻译产物。广泛存在于中枢神经系统,包括前庭传出纤维和耳蜗径路。有神经凋节因子的功能。本文作者以成年雄性 Long-Evans 鼠为研究对象用 CGRP 反转录 RNA(cRNA)同位素~(35)S 标记的探针及 CGRP抗血清相应地在脑干面神经核、Ⅶ副核、耳蜗
The key to studying activity in the nervous system is to understand what neurons play in regulating neurotransmitters and their receptors. DNA recombinant technology provides a guarantee for the study of neuromodulators at the molecular level. In situ hybridization is an important molecular research tool. Calcitonin gene-related peptide (CGRP) is a 37 amino acid polypeptide that is a transcriptional product of calcitonin gene transcription. Widely found in the central nervous system, including vestibular fibers and cochlear pathways. Function of nerve withered factor. In this study, the adult male Long-Evans rats were treated with CGRP-35S-labeled probe and CGRP antiserum, respectively, in the nucleus of the brainstem facial nucleus, Ⅶ nucleus, cochlea