苹果光敏色素作用因子基因PIF的克隆和分析

• 作 者: 宋杨 张艳敏 王传增 刘美艳 刘金 王延玲 陈学森
• 机 构: 山东农业大学作物生物学国家重点实验室,
• 出 处: 园艺学报
• 发表日期: 2012年04期
• 关键词: 光敏色素 PIF 苹果 作用因子 短枝型 短枝 实时荧光定量 结合域 Malus 结构域

以短枝型苹果(Malus domestica Borkh.)为试材,采用RT-PCR结合RACE技术,克隆获得一个光敏色素作用因子(PIF)基因,命名为MdPIF。该基因编码区共2142bp,推测其编码713个氨基酸。氨基酸序列分析显示,在其C端具有保守氨基酸结构域bHLH,N端具有光敏色素结合域APB,与其它植物光敏色素结合因子有很高的同源性。实时荧光定量PCR分析表明,在花后20~110d,MdPIF在短枝型和非短枝型苹果枝条均能表达,同一生长时期,短枝型苹果枝条的表达量显著低于非短枝型,说明苹果短枝性状可能与MdPIF的差异表达有关。MdPIF在叶片、枝条、花瓣、果实和芽中均能表达,在不同器官中的表达量存在明显差异,在枝条中相对表达量最高,推测其可能主要调控苹果枝条的伸长。

Using spruce-type apple (Malus domestica Borkh.) As test material, a phytochrome function factor (PIF) gene was cloned by RT-PCR and RACE technology and named as MdPIF. The gene coding region of a total of 2142bp, presumably encoding 713 amino acids. Amino acid sequence analysis showed that it has a conserved amino acid domain bHLH at its C-terminal and a phytochrome binding domain APB at its N-terminus, which has high homology with other plant phytochrome binding factors. Real-time quantitative PCR analysis showed that MdPIF was able to express in both shoots and non-spiked apple shoots from 20 to 110 days after anthesis. At the same growth stage, MkPIF expression was significantly lower than that of non-spiked shoots, Indicating that apple spur trait may be related to the differential expression of MdPIF. MdPIF was expressed in leaves, shoots, petals, fruits and shoots, and its expression was different in different organs. MdPIF had the highest expression in shoots, suggesting that MdPIF may mainly regulate the elongation of apple shoots.