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目的比较不同时期的胃窦粘膜病理组织中胶原纤维的表达差异。方法选取2013年11月至2014年3月间南方医科大学珠江医院50例胃窦粘膜标本,根据病理结果分为正常对照组(n=6)、溃疡组(n=20)、癌前病变组(n=13)、腺癌组(n=11)。石蜡包埋后切片进行马松(Masson)染色,通过分析其中胶原纤维的沉积面积、累积光密度及平均光密度,比较各组间胶原纤维的表达差异。结果溃疡组、癌前病变组、腺癌组胶原纤维的沉积面积、累积光密度比较,均高于正常对照组(P<0.05),且随着病变严重程度呈逐渐上升趋势,但差异无统计学意义(P沉积面积=0.106;P累积光密度=0.135));平均光密度呈不均衡的“U”型曲线分布,差异有统计学意义(P=0.003)。结论在胃窦粘膜病变中,胶原纤维表达量与疾病的转归具有相关性。
Objective To compare the expression of collagen fibers in the pathological tissues of antral mucosa in different periods. Methods Fifty patients with gastric antral mucosa in Zhujiang Hospital of Southern Medical University from November 2013 to March 2014 were divided into normal control group (n = 6), ulcer group (n = 20), precancerous lesions group (n = 13), adenocarcinoma group (n = 11). Paraffin-embedded sections were stained with Masson. The collagen fiber’s deposition area, cumulative optical density and average optical density were analyzed to compare the expression differences of collagen fibers among the groups. Results The deposition area and cumulative optical density of collagen fibers in ulcer group, precancerous lesion group and adenocarcinoma group were higher than those in normal control group (P <0.05), and there was no statistical difference with the severity of lesion (P = 0.106; P = 0.135)). The average optical density showed an unbalanced “U” curve distribution with a statistically significant difference (P = 0.003). Conclusion In gastric mucosal lesions, the expression of collagen fibers is correlated with the prognosis of the disease.