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为了研究不易感动脉粥样硬化动物北京鸭多种载脂蛋白的基因分子结构，在国内外首次快速构建了鸭肝细胞CDNA文库。采用一步法提取鸭肝细胞总RNA；寡核胸苷酸为吸附介质，柱层析法进一步提取mRNA。以此为模板，利用改良的中CDNA合成方法，反转录合成鸭肝细胞CDNA的第一、二链。经CDNA双链末端修饰，连接含双酶切位点的连接子后重组于噬菌体载体，包装后获得高滴度的鸭肝细胞。CDNA文库，放射自显影显示合成的。CDNA产物大小在400～5000个核苷酸之间，该文库的克隆重组率98．5％以上，随机提取白色噬菌斑DNA，酶切鉴定均有CDNA插入片段。以制备的兔抗鸭aPoAI多抗为探针，从构建的cDNA文库中克隆出鸭aPoAIcDNA核苷酸序列，与常规方法比较，本法简便、快速，易于操作和掌握。本研究还对应用此方法构建CDNA文库的某些环节进行了讨论。
In order to study the molecular structure of various apolipoproteins of atypical animal atypical atheroscintigraphy, CDNA library of duck hepatocytes was constructed rapidly for the first time in China and abroad. Total RNA was extracted from duck hepatocytes by one-step method. Oligothymidylate was used as adsorption medium and further mRNA was extracted by column chromatography. Using this as a template, the first and second strands of CDNA of duck hepatocytes were reverse transcribed by using the modified medium CDNA synthesis method. After double-stranded end-labeling (CDNA) modification of the CDNA, the linker containing the double restriction enzyme site was ligated to the phage vector to obtain high titer duck hepatocytes after packaging. CDNA library, autoradiography shows synthetic. The size of CDNA was between 400 and 5000 nucleotides. The cloning and recombination rate of this library was above 98.5%. The white plaque DNA was randomly extracted and all the cDNA fragments were identified by restriction enzyme digestion. Using the prepared rabbit anti-duck aPoAI polyketide as a probe, the duck aPoAI cDNA nucleotide sequence was cloned from the constructed cDNA library. Compared with the conventional method, the method is simple, rapid, easy to operate and master. This study also discusses some aspects of constructing CDNA libraries using this method.