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AIM:Cyclooxygenase-2(COX-2)has been suggested to beassociated with carcinogenesis.We sought to investigatethe effect of the selective COX-2 inhibitor,Nimesulide onproliferation and apoptosis of SMMC-7721 human hepatomacalls.METHODS:This study was carried out on the culture ofhepatic carcinoma SMMC-7721 call line.Variousconcentrations of Nimesulide(0,200μmol/L、300μmol/L、400μmol/L)were added and incubated.Cell proliferation wasdetected with MTT colorimetric assay,cell apoptosis byelectron microscopy,flow cytometry and TUNEL.RESULTS:Nimesulide could significantly inhibit SMMC-7721cells proliferation dose-dependent and in a dependentmanner compared with that of the control group.Theduration lowest inhibition rate produced by Nimesulide inSMMC-7721 cells was 19.06%,the highest inhibition ratewas 58.49%.After incubation with Nimesulide for 72 h,themost highest apoptosis rate and apoptosis index of SMMC-7721 calls comparing with those of the control were 21.20%±1.62% vs 2.24%±0.26% and 21.23±1.78 vs 2.01±0.23(p<0.05).CONCLUSION:The selective COX-2 inhibitor,Nirnesulide caninhibit the proliferation of SMMC-7721 calls and increaseapoptosis rate and apoptosis index of SMMC-7721 calls.Theapoptosis rate and the apoptosis index are dose-dependent.Under electron microscope SMMC-7721 cells incubated with 300μmol and 400 μmol Nimesulide show apoptotic characteristics.With the clarification of the mechanism of selective COX-2inhibitors,These COX-2 selective inhibitors can become thechoica of prevention and treatment of cancers.
AIM: Cyclooxygenase-2 (COX-2) has been suggested to be associated with carcinogenesis. We sought to investigate the effect of the selective COX-2 inhibitor, Nimesulide onproliferation and apoptosis of SMMC-7721 human hepatomacalls.METHODS:This study was carried out on The culture of hepatic carcinoma SMMC-7721 call line.Various concentrations of Nimesulide (0,200μmol/L, 300μmol/L, 400μmol/L) were added and incubation. Cell proliferation wasdetected with MTT colorimetric assay, cell apoptosis byelectron microscopy, flow cytometry and TUNEL. RESULTS: Nimesulide could significantly inhibit SMMC-7721cells proliferation dose-dependent and in a dependentmanner compared with that of the control group.The duration lowest inhibition rate produced by Nimesulide in SMMC-7721 cells was 19.06%, the highest inhibition rate was 58.49%.After incubation with Nimesulide for 72 h,the most highest apoptosis rate and apoptosis index of SMMC-7721 calls compared with those of the control were 21.20%±1.62% vs 2.24%±0.26% a Nd 21.23±1.78 vs 2.01±0.23 (p<0.05). CONCLUSION: The selective COX-2 inhibitor, Nirnesulide caninhibit the proliferation of SMMC-7721 calls and increase apoptosis rate and apoptosis index of SMMC-7721 calls. Theapoptosis rate and the apoptosis index Are dose-dependent.Under electron microscope SMMC-7721 cells incubated with 300μmol and 400 μmol Nimesulide show apoptotic characteristics.With the clarification of the mechanism of selective COX-2 inhibitors,These COX-2 selective inhibitors can become thechoica of prevention and treatment of cancers .