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应用单个脾结节和性染色体作为天然的细胞遗传学标志,证明存在于纯系LACA或C_(57)胎鼠肝脏和成年小鼠骨髓中的脾结节生成细胞(简称CFU-S)大多具有重建照射小鼠髓系与淋巴系细胞的功能。因而,是一类淋巴-髓系干细胞。然而,存在于正常成年小鼠外周血中的CFU-S,虽然它们也可以在照射小鼠脾脏上生成由各系血细胞组成的脾结节,但其中至少有一部分已经失去了重建照射小鼠造血功能的能力,反映了CFU-S在功能上的不均一性。脾结节测试技术是目前测定造血干细胞的一项重要技术,但是,能够生成脾结节的细胞,不一定都具有造血干细胞的基本特性,一些由造血干细胞分化的多向性造血祖细胞也可能在照射的小鼠脾脏上生成脾结节。
Using single splenic nodules and sex chromosomes as natural cytogenetics markers, it was demonstrated that most of the splenic nodule-producing cells (CFU-S) present in the bone marrow of both pure LACA or C57 (57) fetal and adult mice have Reconstruction of irradiated mouse myeloid and lymphoid cells function. Thus, it is a type of lymphoid-myeloid stem cells. However, CFU-S, which is present in the peripheral blood of normal adult mice, although they can also produce splenic nodules composed of the respective blood cells on the spleens of irradiated mice, at least some of them have lost their ability to reconstitute irradiated mouse hematopoiesis The ability to function reflects the functional inhomogeneity of the CFU-S. Spleen nodules testing technology is currently an important measure of hematopoietic stem cells, but the cells that produce spleen nodules do not always have the basic characteristics of hematopoietic stem cells. Some hematopoietic stem cells may also differentiate into multi-directional hematopoietic progenitor cells Spleen nodules were generated on irradiated spleen of mice.