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本文作者用酚-水法从钩端螺旋体(钩体)哥本哈根血清型芝浦株提取脂多糖(L-LPS),证明具有多种生物学活性,并与大肠杆菌脂多糖(E-LPS)进行了比较,以便了解L-LPS对钩体感染的致病作用。小鼠致死试验:小鼠随机分组,静脉注射7天后,计算LD_(50)。骨髓反应采用吉田法,抗SRBC抗体反应采用Jerne法,活体呑噬试验采用Young改良法。组织病理检查,肠、肺、脾和其他器官的样品以10%福马林固定,石腊包埋切片。血清学检查,显凝试验按WHO方法。抗芝浦株LPS的制备,用LPS与福氏完全佐剂混合皮内免疫家兔。免疫扩散试验以L-LPS作抗原(1mg/ml,每孔为10μl)。结果:(1)L-LPS对小鼠的致死作用,
In this study, the lipopolysaccharide (L-LPS) was extracted from Leptospira serovar Sabouraud (L-LPS) by phenol-water method and was proved to have multiple biological activities and was compared with E. coli lipopolysaccharide (E-LPS) In order to understand the pathogenic role of L-LPS on leptospirosis. Mice Lethality Test: Mice were randomly assigned to LD_ (50) after 7 days of intravenous injection. Yoshida method was used for the bone marrow reaction, Jerne method was used for the anti-SRBC antibody reaction, and the Young modified method was used for the biopsy test. Histopathological examination, samples of intestine, lung, spleen and other organs were fixed with 10% formalin and paraffin embedded. Serological tests, coagulation test according to the WHO method. Anti-Shibu strain LPS preparation, LPS and Freund’s complete adjuvant intradermal immune rabbits. Immunodiffusion Assay L-LPS was used as antigen (1 mg / ml, 10 μl per well). Results: (1) The lethal effect of L-LPS on mice,