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目的观察壳寡糖对肿瘤抑制作用的影响。方法采用体内注射的方法将壳寡糖注入荷瘤小鼠腹腔及瘤内,观察肿瘤生长情况及MTT比色法观察壳寡糖对肿瘤细胞的体外作用。结果壳寡糖对荷瘤小鼠肿瘤的生长有抑制作用,显微镜观察显示肿瘤组织出现坏死;壳寡糖对肿瘤细胞生长有抑制作用,其抑瘤率与浓度有关,与时间无关,细胞经壳寡糖作用后,透射电镜显示细胞有凋亡趋势。结论壳寡糖可抑制肿瘤生长,同时壳寡糖可能诱导肿瘤细胞凋亡。
Objective To observe the effect of chitooligosaccharides on tumor inhibition. Methods The chitooligosaccharides were injected into the peritoneal and intratumoral tumor-bearing mice by the method of in vivo injection. The growth of the tumor was observed and MTT assay was used to observe the effect of chitooligosaccharides on the tumor cells in vitro. Results Chitooligosaccharides inhibited tumor growth in tumor-bearing mice and showed necrosis in tumor tissues under microscope. Chitooligosaccharides inhibited the growth of tumor cells, and the inhibitory rate of chitooligosaccharides was related to concentration, regardless of the time. Oligosaccharides, the transmission electron microscopy showed that the cells have apoptosis trends. Conclusion Chitooligosaccharides can inhibit tumor growth, while chitooligosaccharides may induce tumor cell apoptosis.