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建立了碘的反相高效液相色谱测定方法。色谱条件为：Ｓｈｉｍ－Ｐａｃｋ ＣＬＣ－ＯＤＳ柱；流动相为甲醇－水（２０：８０）；流速为１ｍＬ／ｍｉｎ；检测波长为２９０ｎｍ。本方法的线性范围为０．１０～１０ ｍｇ／Ｌ；相对标准偏差为１．１％～１．４％；加标回收率为９７％～１０１％。所建立的方法已用于医用碘酒的测定。
A method for the determination of iodine by reversed-phase high performance liquid chromatography was established. The chromatographic conditions were as follows: Shim-Pack CLC-ODS column; the mobile phase was methanol-water (20:80); the flow rate was 1 mL / min; the detection wavelength was 290 nm. The linear range of this method was 0.10 ~ 10 mg / L; the relative standard deviation was 1.1% ~ 1.4%; the recoveries were 97% ~ 101%. The established method has been used for the determination of medical iodine.