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该文研究了次氯酸钠氧化的低密度脂蛋白(oxidized low-density lipoprotein,oxLDL)对肾上腺醛固酮分泌的影响。方法 :取自健康志愿者的LDL[自然LDL(NatLDL)]并在体外进行氧化修饰。应用NatLDL和oxLDL刺激NCI-H295R细胞,通过放射免疫法测量醛固酮释放的水平。采用免疫印迹法和定量RT-PCR分析分子结构的变化。结果 :NatLDL和oxLDL引起剂量依赖性增加性
This article investigates the effect of oxidized low-density lipoprotein (oxLDL) on adrenal aldosterone secretion. Methods: LDL [NatLDL] from healthy volunteers and oxidative modification in vitro. NCI-H295R cells were stimulated with NatLDL and oxLDL, and the level of aldosterone release was measured by radioimmunoassay. Western blotting and quantitative RT-PCR analysis of molecular structure changes. Results: NatLDL and oxLDL caused a dose-dependent increase