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对烟草品种9种同工酶进行了聚丙烯酰胺凝胶电泳分析，苹果酸酶（苗期叶部1个位点，花蕾1个位点）、6-磷酸葡萄糖酸脱氢酶（苗期叶部2个位点）、乳酸脱氢酶（大田旺长期叶1个位点）、过氧化物酶（旺长期叶部6个位点，花蕾4个位点）、酯酶（现蕾期叶部1个位点，花蕾1个位点）5个酶的17个位点品种间存在多态性，而淀粉酶、乙醇脱氢酶、酸性磷酸酶、苹果酸脱氢酶品种间无差异。据5种同工酶17个多态性位点计算的遗传距离对品种进行分类，各群基本由来源国家相同的品种构成。同工酶遗传距离与产量杂优无相关关系，但群间品种杂交比群内品种杂交更易获得杂优，可用同工酶遗传距离构建品种杂种优势群。
Nine isozyme isozymes of tobacco cultivars were analyzed by polyacrylamide gel electrophoresis. The content of malic enzyme (one locus in seedling and one flower bud), gluconate dehydrogenase (seedling leaf 2 loci), lactate dehydrogenase (1 locus in the leaves of longtianwang), peroxidase (6 locus in the long and medium leaves, 4 locus of flower buds), esterase 1 locus and 1 locus of flower buds) had polymorphisms among 17 loci of 5 enzymes, but there was no difference among amylase, alcohol dehydrogenase, acid phosphatase and malate dehydrogenase. According to the genetic distance calculated by 17 polymorphic loci of 5 isozymes, the cultivars were classified, and each group consisted of the same varieties from the same country of origin. The isozyme isozymes had no correlation with the yield heterosis, but heterosis was more likely to be obtained by crossbreeding among the crossbreeds than that within the crossbreeds, and the heterotic population could be constructed with isozyme genetic distance.