对三种不同型丙型肝炎病毒感染的血清学应答

• 作 者: 陈燕
• 出 处: 国外医学.预防.诊断.治疗用生物制品分册
• 发表日期: 1992年05期
• 关键词: 丙型肝炎病毒 血友病患者 酶免疫试验 基因序列 血清学反应 非甲非乙型肝炎 序列同源性 免疫印迹法 献血员 非编码区

基因序列的变异性对酶免疫试验(EIA)诊断丙型肝炎病毒(HCV)的影响仍难以确定。随着2型HCV的发现,这个问题变得更重要了,2型HCV的基因序列与1型HCV有很大不同,且分布于全世界。作者比较了献血者、血友病患者和静注毒品者的5′端非编码区(5′NCR)、核心、NS-3和NS-5区扩增的序列,发现对应于1型和2型变异体,有两组高度变异的序列。作者还发现第3组HCV变异体,暂称3型,其序列的变异程度

The impact of gene sequence variability on the diagnosis of hepatitis C virus (HCV) by enzyme immunoassay (EIA) remains unclear. This problem becomes even more important with the discovery of type 2 HCV, which is very different from that of type 1 HCV and is distributed throughout the world. The authors compared the sequence of the 5 ’untranslated region (5’NCR), core, NS-3 and NS-5 regions of blood donors, hemophiliacs and intravenous drug users and found that they correspond to type 1 and type 2 Type variants, two highly variable sequences. The authors also found that the third group of HCV variants, tentatively called type 3, the degree of variation of its sequence