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采用ＰＣＲ技术分析中国汉族、德国人、斯洛伐克人和美国黑人群体Ｄ１９Ｓ４００基因座的遗传多态性及世界三大人种之间的差异。四个群体共调查了６２０人，发现了１１个等位基因，观察到４７种基因型。各群体观察杂合度为：０．７８～０．８８，个人识别机率为：０．９３８５０～０．９６６４。四个群体基因型频率分布符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡（Ｐ＞０．０５），三大人种（蒙古人种、高加索人种、美国黑人）之间Ｄ１９Ｓ４００基因座等位基因频率分布存在极显著差异（Ｐ＜０．０１）。结果显示Ｄ１９Ｓ４００基因座在群体遗传学研究和法医学个人识别中有较高应用价值
The genetic polymorphisms of the D19S400 locus in Chinese Han, German, Slovak and Afro-American populations and the differences among the three major human races in China were analyzed by PCR. Of the four groups, 620 were surveyed, 11 alleles were found and 47 genotypes were observed. The observed heterozygosity of each group was 0.78 ~ 0.88, and the probability of personal identification was 0.93850 ~ 0.9664. The frequency distribution of genotypes of the four groups was in accordance with the Hardy-Weinberg equilibrium (P> 0.05). There was a significant difference in allele frequencies of the D19S400 locus between the three races (Mongolian, Caucasians, African Americans) P <0.01). The results showed that the D19S400 locus has high value in population genetics and forensic personal identification