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荧光胺是一种非荧光剂,易与伯胺反应形成荧光产物,被普遍用于伯胺的荧光光谱定量分析.本文利用荧光胺与伯胺反应发展了一种新型灵敏的伏安法用于检测水溶液中的伯胺.首先,在有、无伯胺的0.1 mol.L-1PBS(pH 9.0)缓冲液中,研究了玻碳电极表面荧光胺的循环伏安电化学行为.荧光胺的不可逆氧化峰出现在0.70 V(vs.SCE),当加入伯胺时,在0.46 V(vs.SCE)出现另一不可逆的氧化峰,为荧光胺与伯胺反应的产物.继续加入氨水,荧光胺的氧化峰变弱,反应产物的氧化峰则由于荧光胺按反应化学计量比随氨消耗增多而随之增大.上述两个阳极峰分别对应于荧光胺及其反应产物,采用方波伏安和荧光光谱技术可实现水溶液中伯胺的定量检测.在0～60μmol.L-1氨浓度范围内,该反应产物方波伏安检测成线性响应.S/N=3或3σ时检测下限分别为0.71μmol.L-1和3.17μmol.L-1,与荧光法检测的结果相近.
Fluorescent amine is a non-fluorescent agent, easy to react with primary amines to form fluorescent products, and is widely used in quantitative analysis of primary amine fluorescence spectra.In this paper, a new sensitive voltammetric method was developed for the The primary amines in aqueous solution were detected firstly.Firstly, the cyclic voltammetry of fluorescent amines on the surface of glassy carbon electrode was investigated in 0.1 mol·L-1 PBS (pH 9.0) with or without primary amines.The irreversible The oxidation peak appeared at 0.70 V (vs.SCE) and another irreversible oxidation peak appeared at 0.46 V (vs.SCE) as the product of the reaction of the fluorescamine with the primary amine when added to the primary amine. Continuing the addition of aqueous ammonia, the fluorescamine Of the oxidation peak becomes weaker and the oxidation peak of the reaction product increases due to the increase of the stoichiometry of the fluorescent amine with the consumption of ammonia.The above two anodic peaks correspond to the fluorescent amine and the reaction product thereof respectively and the square wave voltammetry And fluorescence spectroscopy can be used for the quantitative detection of primary amines in aqueous solution.The square wave voltammetry of the reaction product was linearly detected in the range of 0 ~ 60μmol.L-1 ammonia concentration.The detection limit was lower at S / N = 3 or 3σ 0.71μmol.L-1 and 3.17μmol.L-1, which were similar to the results of fluorescence method.