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1952年Gross和Pitt-Rivers首先注意到在人的血清中有3,5,3′—三碘—L—甲腺原氨酸(T_3)。几年以后,Pitt-Pivezs等阐明在无甲状腺功能的粘液性水肿病人给甲状腺素(T_4)后可产生T_3。1957年Maclagen等第一次报告了“T_3甲状腺中毒”——血浆T_3浓度增高和T_4浓度正常的毒性甲状腺肿的病例。1969年以来,由于对T_3生物化学测定方法的改进,人们才广泛认识到T_3对健康和疾病的作用,并促进了可靠和敏感的放射免疫测定方法的发展。近几年,关予T_3的作用、代谢和临床重要性的研究迅速增多,本文尝试对这些新的文献进行综述。 T_3的测定测定T_3的化学方法基本包括三个步骤:(1)从血浆蛋白中分离出与其正常结合的甲状腺激素(T_3和T_4);(2)纸层析法;(3)竞争性蛋白结合或饱和分析测定。所有测定T_3的化学方法都比较困难,费时和不准确,但这些方法使人们对T_3在甲状腺激素生理作用中的重要性引起了重视并促进了代替化学测定
In 1952, Gross and Pitt-Rivers first noticed 3,5,5’-triiodo-L-thyronine (T_3) in human serum. Several years later, Pitt-Pivezs et al. Demonstrated that T_3 can be produced in patients with thyroid-free myxedema by administering thyroxine (T_4). In 1957, Maclagen et al reported for the first time that “T_3 thyrotoxicosis” - increased plasma T_3 concentration and T_4 concentration of normal cases of toxic goiter. Since 1969, improvements in T_3 biochemistry have led to widespread recognition of the effects of T_3 on health and disease and the development of reliable and sensitive radioimmunoassay methods. In recent years, the role of T_3, metabolic and clinical significance of the rapid increase in the study, this article attempts to review these new literature. Determination of T_3 The chemical method for the determination of T_3 basically consists of three steps: (1) separation of thyroid hormones (T_3 and T_4) from normal plasma protein from plasma proteins; (2) paper chromatography; (3) competitive protein binding Or saturation analysis. All of the chemical methods used to determine T_3 are difficult, time consuming and inaccurate, but these methods have given rise to the importance of T_3 in the physiological role of thyroid hormones and have promoted the use of alternative chemical assays