Study of Oxidation of Glutathione Treated with Hypochlorous Acid by Capillary Electrophoresis

• 出 处: Chinese Chemical Letters
• 发表日期: 2001年07期
• 关键词: Glutathione reversible GSSG ammonium glutathione capillary oxidized minutes pero

Capillary electrophoresis (CE) method was developed for the separation and quantification of reduced glutathione (GSH), oxidized glutathione (GSSG) and glutathione sulphonic acid (GSO_3H). Baseline separation was obtained within five minutes. The effects of reaction time and molar ratio of hypochlorous acid (HOCl) to GSH on the oxidation of GSH were investigated.

Capillary electrophoresis (CE) method was developed for the separation and quantification of reduced glutathione (GSH), oxidized glutathione (GSSG) and glutathione sulphonic acid (GSO_3H). The effects of reaction time and molar ratio of hypochlorous acid (HOCl) to GSH on the oxidation of GSH were investigated.