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目的 :探讨分析结肠癌患者外周血淋巴细胞与结肠癌组织内谷胱甘肽硫转移酶 (GST)活性变化及二者的相互关系 ,评估结肠癌患者外周血淋巴细胞内GST活性变化在结肠癌发生发展过程中的临床价值。方法 :应用1-氯 - 2 ,4-二硝基苯为底物的比色分析法 (34 0nm) ,对 31例结肠癌患者外周血淋巴细胞和癌组织内GST活性变化及二者之间的关系进行了分析。结果 :1)结肠癌患者较正常人淋巴细胞内GST活性明显升高 ,分别为 2 83±2 0 4和 96± 14 3(P <0 0 1)。 2 )结肠癌组织较正常临近组织内GST活性明显增高 ,分别为 14 7± 2 2 9和 37±10 8(P <0 0 1)。 3)结肠癌患者外周血淋巴细胞内与癌组织内GST活性变化具有良好的相关性 (r =0 80、P <0 0 1)。 4)正常人及结肠癌患者淋巴细胞内GST活性变化相对稳定 ,分别在 89 3～ 110 6和 2 5 9 9～ 310 4之间波动。 5 )未转移结肠癌患者外周血淋巴细胞内GST活性在手术一周后基本恢复正常。 6 )结肠癌患者外周血淋巴细胞内GST活性变化与性别、年龄及肿瘤的临床分期无关。结论 :这些研究提示作为肿瘤标记物的GST ,其在外周血淋巴细胞内的活性变化相对稳定 ,并且与结肠癌组织内GST活性变化成正相关。因此结肠癌患者外周血淋巴细胞内GST活性的升高变化可作为结肠癌早期发现
OBJECTIVE: To investigate the changes of glutathione-S-transferase (GST) activity in peripheral blood lymphocytes and colon cancer in patients with colon cancer, and to explore the relationship between GST activity in peripheral blood lymphocytes and colorectal cancer patients. The clinical value in the development process. METHODS: The colorimetric assay (34 nm) using 1-chloro-2,4-dinitrobenzene as a substrate was used to detect changes in GST activity in peripheral blood lymphocytes and cancer tissues from 31 patients with colon cancer and between the two groups. The relationship was analyzed. RESULTS : 1) The activity of GST in lymphocytes of patients with colon cancer was significantly higher than that in normal individuals, with 2 83 ± 2 0 4 and 96 ± 14 3, respectively (P <0 01). 2) The activity of GST in colorectal cancer tissue was significantly higher than that in the normal adjacent tissues, which were 14 7 ± 2 29 and 37 ± 10 8 (P <0 01). 3) There was a good correlation between GST activity in peripheral blood lymphocytes and cancer tissues in patients with colon cancer (r = 80, P <0 01). 4) The changes of GST activity in lymphocytes of normal people and colon cancer patients were relatively stable and fluctuate from 89 3 to 110 6 and 2 5 9 9 to 310 4 respectively. 5) The activity of GST in peripheral blood lymphocytes of patients with non-metastatic colon cancer basically returned to normal one week after surgery. 6) The change of GST activity in peripheral blood lymphocytes of patients with colon cancer has nothing to do with gender, age and clinical stage of tumor. Conclusion: These studies suggest that GST as a tumor marker has a relatively stable activity in peripheral blood lymphocytes and is positively correlated with changes in GST activity in colon cancer tissues. Therefore, the increase of GST activity in peripheral blood lymphocytes of colon cancer patients can be used as early detection of colon cancer.