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目的：研究经口补充谷氨酰胺（Ｇｌｕｔａｍｉｎｅ，Ｇｌｎ）对创伤大鼠细胞免疫功能的影响。方法：采用闭合性创伤大鼠模型，通过补充或不补充谷氨酰胺，观察创伤后血浆、脾脏和肠系膜淋巴结细胞Ｇｌｎ含量，以及细胞免疫功能的动态变化。结果：创伤后补充Ｇｌｎ组（Ｇｌｎ组）血浆、脾脏和肠系膜淋巴结细胞Ｇｌｎ含量明显高于不补充Ｇｌｎ组（Ｎｏｎ－Ｇｌｎ组）；伤后大鼠脾脏和肠系膜淋巴结细胞的Ｔ淋巴细胞增殖反应下降，白介素－２（ＩＬ－２）生成减少，细胞膜ＩＬ－２受体（ＩＬ－２Ｒ）表达受抑，但Ｇｌｎ组动物的免疫抑制程度明显轻于Ｎｏｎ－Ｇｌｎ组。结论：经口补充Ｇｌｎ，能有效地提高创伤后血浆Ｇｌｎ水平，保持脾脏、淋巴结细胞及腹腔巨噬细胞内Ｇｌｎ正常含量，改善细胞免疫功能，减轻创伤后的免疫受抑程度。
Objective: To investigate the effect of oral glutamine (Gln) on cellular immune function in traumatic rats. Methods: The model of traumatic injury in rats was established. Gln levels in plasma, spleen and mesenteric lymph nodes of wounds and dynamic changes of cellular immune function were observed with or without glutamine. Results: Gln levels in plasma, spleen and mesenteric lymph node cells in Gln group (Gln group) were significantly higher than those in Gln group (Non-Gln group) after wounding. Injury of T lymphocytes in spleen and mesenteric lymph node decreased IL-2 and IL-2R, but the immunosuppression in Gln group was significantly lower than that in Non-Gln group. CONCLUSION: Oral administration of Gln can effectively increase the post-traumatic plasma Gln levels, maintain normal Gln levels in spleen, lymph node cells and peritoneal macrophages, improve cellular immune function and relieve post-traumatic immune suppression.