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目的检测核糖核酸酶抑制因子(Ribonuclease Inhibitor,RI)基因在人结肠癌及同体切缘组织中的表达情况,初步探讨RI与结肠癌生长及临床分期之间的关系。方法分别使用Western blotting法和RT-PCR法,检测28例结肠癌组织、切缘组织中RI蛋白和RI mRNA的表达情况。结果结肠癌组织中RI蛋白及mRNA的表达均明显低于切缘组织,不同临床分期之间的表达无明显差别。结论 RI的表达下降可能与结肠癌生长有关,但与结肠癌的临床分期无关。
Objective To detect the expression of Ribonuclease Inhibitor (RI) gene in human colorectal carcinoma and its adjacent tissues, and to explore the relationship between RI and the growth and clinical stage of colon cancer. Methods Western blotting and RT-PCR were used to detect the expression of RI and RI mRNA in 28 cases of colorectal cancer tissues and marginal tissues. Results The expression of RI protein and mRNA in colon cancer tissues was significantly lower than that in the marginal tissues. There was no significant difference between different clinical stages. Conclusion The decreased expression of RI may be related to the growth of colon cancer, but not to the clinical stage of colon cancer.