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The enhancing effect of benserazide on the chemiluminescence of luminol-potassium ferricyanide in alkaline medium was investigated, and a sensitive method for detecting benserazide has been developed by flow-injection chemiluminescences. The experimental results showed that the emission intensity is proportional to the concentration of benserazide in the range of 0.01-1.0 μg/mL. The detection limit (S/N=3) for benserazide turned out to be 0.9 ng/mL, and the relative standard deviation for 11 determinations of a 0.10 μg/mL benserazide was 2.4%. The method was satisfactorily applied to the determination of benserazide in spiked urine samples.