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目的:观察线栓法致大鼠局灶性脑缺血模型后,不同时相神经功能评分、脑梗死灶体积、血清神经元特异性烯醇化酶(neuron specific enolase,NSE)含量的变化。方法:大鼠随机分为脑缺血模型组和假手术组,每组大鼠又随机分为缺血1、6、12、24、72 h组与120 h组6个时相组。采用线栓法建立大鼠局灶性脑缺血模型,观察记录大鼠大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)后不同时相神经功能评分、测定血清NSE含量的变化,TTC染色观察脑梗死灶体积。结果:假手术组神经功能评分均为0;模型组在缺血1 h神经功能评分就明显升高,24 h达到最高,持续到120 h。假手术组脑梗死灶体积均为0;模型组在缺血6 h脑梗死灶体积才明显升高,24 h达到最高,72 h明显下降,120 h再次升高。假手术组血清NSE含量在各个时相没有明显变化;模型组在缺血1 h血清NSE含量就明显升高,12 h达到最高,24 h明显下降,一直持续到120 h。结论:脑缺血后神经功能评分、脑梗死灶体积、血清中NSE含量都明显升高,但三者在不同时相的变化是不一致的。
OBJECTIVE: To observe the changes of neural function score, volume of cerebral infarction and the content of serum neuron specific enolase (NSE) after focal cerebral ischemia model induced by suture suppository in rats. Methods: The rats were randomly divided into cerebral ischemia model group and sham operation group. Each group of rats were randomly divided into six phase groups of 1, 6, 12, 24, 72 h and 120 h groups. The model of focal cerebral ischemia in rats was established by thread occlusion. The neurological function scores of the rats at different phases after middle cerebral artery occlusion (MCAO) were recorded, and the changes of serum NSE levels were measured. TTC staining was used to observe the brain Infarct volume. Results: The scores of neurological function in sham operation group were all 0. The score of neurological function in model group at 1 h after ischemia increased significantly, reached the peak at 24 h and lasted for 120 h. The volumes of cerebral infarction in the sham operation group were all 0. The volume of cerebral infarction in the model group was significantly increased at 6 h after ischemia, reached the peak at 24 h, decreased significantly at 72 h and then increased again at 120 h. The serum NSE level in the sham operation group did not change significantly at all time points. In the model group, the serum NSE level increased significantly at 1 h after ischemia, reaching the highest level at 12 h and decreasing significantly at 24 h, continuing until 120 h. CONCLUSION: The score of neurological function, the volume of cerebral infarction and the content of NSE in serum are obviously increased after cerebral ischemia, but the changes of the three in different phases are inconsistent.