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A novel variant of human vascular endothelial growth factor (h’VEGF165) cDNA was amplified by nested PCR method from the HL60 1 cells and was cloned into a eukaryotic expressing vector pcDNA 3 to construct a recombinant plasmid pCD-h’VEGF165. The amplified h’VEGF165 cDNA fragment was identified by enzyme digestion and DNA sequencing methods. Also, wild-type hVEGF165 cDNA was obtained, identified and cloned into a eukaryotic expressing vector pcDNA3 by using the same methods. The results of DNA sequencing showed that h’VEGF165 cDNA cloned from HL60 1 was 600 bp in size with 8 % of the base sequence in h’VEGF165 cDNA being changed as compared with the base sequence in the wild-type hVEGF165 cDNA. The results of sequencing of hVEGF165 which was cloned from HL60 by us were consistent with the reports completely.
A novel variant of human vascular endothelial growth factor (h’VEGF165) cDNA was amplified by nested PCR method from the HL60 1 cells and was cloned into an eukaryotic expression vector pcDNA 3 to construct a recombinant plasmid pCD-h’VEGF165. The amplified h The VEGF165 cDNA fragment was identified by enzyme digestion and DNA sequencing methods. Also, wild-type hVEGF165 cDNA was obtained, identified and cloned into an eukaryotic expression vector pcDNA3 by using the same methods. The results of DNA sequencing showed that h’VEGF165 cDNA cloned from HL60 1 was 600 bp in size with 8% of the base sequence in h’VEGF165 cDNA being changed as compared with the base sequence in the wild-type hVEGF165 cDNA. The results of sequencing of hVEGF165 which was cloned from HL60 by us were consistent with the reports completely.