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Objective To investigate the expression of B cell activation factor of the TNF family(BAFF) gene between human normal and inflamed fallopian tubes.Methods Tissue samples of human normal fallopian tube(n=20) and inflamed fallopian tube(n=20) were collected. The expression of BAFF gene was determined by the realtime reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry.Results BAFF immunostaining appeared on the cellular membrane and in the cytoplasm of tubal epithelial cells. Both BAFF protein and mRNA in normal fallopian tubes had lower levels than those in inflamed fallopian tubes(P<0.01).Conclusion BAFF protein and mRNA are present in human tubal tissues. BAFF gene in human inflamed fallopian tube would have a high expression.
Objective To investigate the expression of B cell activation factor of the TNF family (BAFF) gene between human normal and inflamed fallopian tubes. Methods Tissue samples of human normal fallopian tube (n = 20) and inflamed fallopian tube (n = 20) were collected . The expression of BAFF gene was determined by the real time reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Results BAFF immunostaining was performed on the cellular membrane and in the cytoplasm of tubal epithelial cells. Both BAFF protein and mRNA in normal fallopian tubes had lower levels than those in inflamed fallopian tubes (P <0.01) .Conclusion BAFF protein and mRNA are present in human tubal tissues. BAFF gene in human inflamed fallopian tube would have a high expression.