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目的：观察银杏叶提取物（Ｇｉｎｋｇｏ ｂｉｌｏｂａ ｅｘｔｒａｃｔ，ＧｂＥ）对培养新生牛脑微血管内皮及平滑肌细胞粘附单 核细胞及中性粒细胞的影响。方法：用ＴＮＦ－α、ＩＬ－６诱导传代的新生牛脑微血管内皮（ＣＭＥＣ）及平滑肌细胞， 粘附大鼠血单核细胞（ ＭＮＣ）及中性粒细胞（ＰＭＮ），计算粘附率。结果： ＣＭＥＣ和 ＣＭＳＭＣ经 ＴＮＦ－ α和 ＩＬ－ ６处理 后，增加ＭＮＣ，ＰＭＮ粘附到内皮和平滑肌细胞表面达加入细胞总数的３０～６０％，（对照组小于２０％），ＧｂＥ可剂 量依赖性地抑制 ＴＮＦ－α和 ＩＬ－ ６的诱导作用。结论：ＧｂＥ可抑制 ＣＭＥＣ，ＣＭＳＭＣ和 ＰＭＮ，ＭＮＣ的粘附。
Objective: To observe the effects of Ginkgo biloba extract (GbE) on the adhesion of mononuclear cells and neutrophils to cultured neonatal bovine cerebral microvascular and smooth muscle cells. METHODS: Neonatal bovine cerebral microvascular endothelial (CMEC) and smooth muscle cells, adherent rat blood mononuclear cells (MNCs) and neutrophils (PMNs) were passaged with TNF-α and IL-6, and the adhesion rate was calculated. Results: After CMEC and CMSMC were treated with TNF-α and IL-6, MNC and PMN adhered to the surface of endothelium and smooth muscle cells to 30 to 60% of the total number of added cells (less than 20% in the control group). GbE was dose-dependent Inhibits the induction of TNF-α and IL-6. Conclusion: GbE can inhibit the adhesion of CMEC, CMSMC and PMN, MNC.