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以11个木薯品种(系)(SC205、SC5、SC8、Arg7、Rayong9、KU50、9I、R3、17Q、FB-1、FB-4)为材料,采用田间统计分析,实时定量PCR和皮尔森相关分析,对不同木薯品种(系)的生物积累量特性、淀粉积累关键基因的表达及两者之间的相关性进行分析。结果表明,品种(系)间在叶绿素含量、净光合速率、干物质积累率及相关途径重要基因表达水平上均存在显著差异。其中:块根产量高的品种或品系FB-1、SC5、SC8、KU50、SC205、Rayong9在块根成熟期保持较高的干物质积累量;而对于叶片叶绿素含量和净光合速率,SC205保持最高,品种(系)间存在差异(P<0.05),但与产量没有明显相关性;块根膨大期叶片净光合速率最高,且与叶绿素含量呈显著正相关。基因表达与产量性状的相关分析表明,块根形成期MeAGPS1的表达量及块根形成期和膨大期MeGBSS1的表达量与块根膨大期木薯块根的干物质率呈显著正相关,块根成熟期MeSBE2.2的表达量与块根成熟期木薯块根的干物质率呈显著正相关。
A total of 11 cassava varieties (SC205, SC5, SC8, Arg7, Rayong9, KU50, 9I, R3, 17Q, FB-1 and FB-4) were used as materials. Field statistical analysis, real-time PCR and Pearson correlation Analysis of the bioaccumulation characteristics of different cassava varieties (lines), the expression of key genes of starch accumulation and the correlation between the two. The results showed that there were significant differences in the chlorophyll content, net photosynthetic rate, the rate of dry matter accumulation and the expression of important genes in related pathways among varieties (lines). Among them, FB-1, SC5, SC8, KU50, SC205 and Rayong9 maintained high dry matter accumulation at maturity of root tuber, while SC205 maintained the highest level of leaf chlorophyll content and net photosynthetic rate. (Lines) (P <0.05), but had no significant correlation with yield. The net photosynthetic rate of tuberous leaves in the bulking stage was the highest, and it was positively correlated with the content of chlorophyll. Correlation analysis of gene expression and yield traits showed that there was a significant positive correlation between the expression level of MeAGPS1 and the expression of MeGBSS1 in tuberous root formation stage and the dry matter content of cassava root in tuberous expansion stage. There was a significant positive correlation between the expression level and the dry matter content of cassava roots in the mature root tuberization.