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本文报告观察了不同个体淋巴细胞对环磷酰胺和胸腺素的反应性及在不同实验条件下药物效应的差异,结果初步提示,机体免疫状态的差异影响机体对药物免疫调节效应的反应性。 材料与方法 分裂原反应性测定 本室建立的微量全血法。肝素抗凝血种入96孔微量培养板(20μl/孔),再加入分裂原液(PHA,广州医工所)及受试药物各0.1ml,37℃,5％CO_2培养72h,~3H·TdR掺入6h后收获,液体闪烁计数仪(LKB)测cpm值。结果表示为净cpm=cpm(分裂原刺激孔)-cpm(自发转化孔)。分裂原及药物均以RPMI1640(不含血清)液配制。
In this paper, we observed the reactivity of different individuals’ lymphocytes to cyclophosphamide and thymosin and the differences in drug effects under different experimental conditions. The results suggest that the difference of immune status affects the response of the body to the immunomodulatory effects of drugs. Materials and Methods Determination of mitogenic reactivity established in this room micro-whole blood method. Heparin anticoagulant blood was seeded into 96-well microplates (20 μl / well), and 0.1 ml each of the split stock solution (PHA, Guangzhou Medical Research Institute) and the test drug was added thereto. The cells were incubated at 37 ° C, 5% CO 2 for 72 h, Harvested after 6h harvest, liquid scintillation counter (LKB) measured cpm value. The results are expressed as net cpm = cpm (mitogen-stimulated pores) -cpm (spontaneous transformation pore). The original split and drugs were formulated with RPMI1640 (serum-free) solution.