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以籼稻品种9311为遗传背景携带日本晴置换片段的119个染色体片段置换系(CSSL)为试验材料,以相对根长为耐性指标,利用代换作图方法对水稻苗期耐Fe2+胁迫QTL进行了定位。结果表明,以P≤0.01为阈值,筛选到相对根长与受体亲本9311差异极显著的14个CSSL,共鉴定了9个耐Fe2+胁迫QTL,分布于水稻第2、5、6、7和12染色体上,其中4个QTL在含1个重叠片段的CSSL中被检测到,其余5个QTL在含2～3个重叠片段的CSSL中被检测到。9个耐Fe2+胁迫QTL中,qRRL12的加性效应值为负,效应来源于对Fe2+胁迫敏感的亲本9311,其余8个QTL的加性效应值为正,其加性效应值变化范围为7.63～19.88,加性效应百分率的变化范围为13.69%～35.66%,效应均来源于耐Fe2+胁迫的亲本日本晴。
A total of 119 chromosomal fragment substitution lines (CSSLs) harboring Nipponbare as a genetic background in indica rice variety 9311 were selected as experimental materials. The relative root length was used as tolerance index to map QTLs resistant to Fe2 + stress in rice at seedling stage. . The results showed that 14 CSSLs with significant difference between relative root length and recipient 9311 were screened with P≤0.01 as threshold, and 9 QTLs resistant to Fe2 + stress were identified and distributed on rice 2, 5, 6, 7 and On chromosome 12, four QTLs were detected in CSSL with one overlapping fragment and the remaining five QTLs were detected in CSSL with two to three overlapping fragments. Among 9 resistant QTLs, the additive effect of qRRL12 was negative, and the effect was derived from 9311 sensitive to Fe2 + stress. The additive effects of the other 8 QTLs were positive and their additive effects ranged from 7.63 ~ 19.88, and the percentage of additive effect ranged from 13.69% to 35.66%. The effects were all from Nipponbare, the parent of Fe2 + stress resistance.