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[Objective] To observe the effects of total flavonoids in Drynaria fortunei (Kunze) J. Sm. (TFDF) on osteoblasts (OB) differentiation and the expression of p38 MAPK and BMP-2/Smads signaling in osteoblasts after treatment by Advanced Glycation End Products (AGEs), so as to explore the action mechanism for preventing and curing osteoporosis of TFDF. [Method] Primary osteoblasts of newborn Sprague-daweley rats were extracted and cultured by the double enzyme digestion method, and its biological characteristics were observed. pNPP, ELISA and Alizarin dyeing were respectively used to test ALP, Type I collagen (Col I), osteocalcin (BGP), BMP-2, and mineralization of osteoblasts after treatment by different concentration of AGEs and after treatment by different concentration of TFDF and AGEs (400 mg/L). Western-blot was used to detect p38 and Smad1/5/8 protein phosphorylation after treatment by TFDF and AGEs (400 mg/L). [Result] The levels of ALP, Type I collagen, osteocalcin and mineralization in osteoblasts treated by different concentrations of AGEs (200, 400 and 800 mg/L) were lower than those in the control group. TFDF could increase ALP, Type I collagen, osteocalcin, BMP-2 and mineralization of osteoblasts in a dose-dependent manner after treatment by AGEs (400 mg/L). TFDF (50 mg/L) can increase protein phosphorylation of p38 and Smad1/5/8 in osteoblasts after treatment by AGEs (400 mg/L). [Conclusion] AGEs can decrease differentiation and mineralization of osteoblasts. TFDF can increase differentiation and mineralization of osteoblasts in a dose-dependent manner after treatment by AGEs, which may be related to p38 and BMP-2/Smads signaling.
(TF) on osteoblasts (OB) differentiation and the expression of p38 MAPK and BMP-2 / Smads signaling in osteoblasts after treatment by Advanced Glycation End Products (AGEs), so as to explore the action mechanism for preventing and curing osteoporosis of TFDF. [Method] Primary osteoblasts of newborn Sprague-daweley rats were extracted and cultured by the double enzyme digestion method, and its biological characteristics were observed. PNPP , ELISA and Alizarin dyeing were respectively used to test ALP, Type I collagen (Col I), osteocalcin (BGP), BMP-2, and mineralization of osteoblasts after treatment by different concentration of AGEs and after treatment by different concentration of TFDF and AGEs (400 mg / L) Western-blot was used to detect p38 and Smad1 / 5/8 protein phosphorylation after treatment by TFDF and AGEs (400 mg / L). [Result] The levels of ALP, Type I collagen, osteocalcin and mineralizat ion in osteoblasts treated with different concentrations of AGEs (200, 400 and 800 mg / L) were lower than those in the control group. TFDF could increase ALP, Type I collagen, osteocalcin, BMP-2 and mineralization of osteoblasts in a dose- dependent manner after treatment by AGEs (400 mg / L). TFDF (50 mg / L) can increase protein phosphorylation of p38 and Smad1 / 5/8 in osteoblasts after treatment by AGEs decrease differentiation and mineralization of osteoblasts. TFDF can increase differentiation and mineralization of osteoblasts in a dose-dependent manner after treatment by AGEs, which may be related to p38 and BMP-2 / Smads signaling.