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目的:采用从成品图谱逆向回溯至原料药材的方式,研究消银解毒袋煎剂HPLC-DAD指纹图谱,建立成品整体特征的质量评价方法。方法:采用HPLC-DAD检测,色谱条件:Dikma Platisil ODS C18(250 mm×4.6 mm,5μm);流动相:乙腈-(0.1%磷酸-0.1%三乙胺溶液),梯度洗脱;柱温30℃。结果:建立的HPLC-DAD指纹图谱中,16个色谱峰分别归属到处方中黄芩、鱼腥草、枳壳、紫草、甘草5个药材,4个色谱峰可明确化学成分。结论:建立的HPLC-DAD指纹图谱表达了消银解毒袋煎剂可测组分的整体特征,提高了对中药复方质量控制的专属性,逆向回溯的研究模式扩展了大复方质量研究的思路。
OBJECTIVE: To study HPLC-DAD fingerprints of Yin-Yin detoxification bag decoction and to establish the quality evaluation method for the overall characteristics of the finished product by backtracking it from the finished product map to the raw medicinal materials. Methods: HPLC-DAD was used for the determination. The chromatographic conditions were as follows: Dikma Platisil ODS C18 (250 mm × 4.6 mm, 5 μm); gradient elution with acetonitrile- (0.1% phosphoric acid- 0.1% triethylamine) ℃. Results: The established HPLC-DAD fingerprints, 16 chromatographic peaks were assigned to the prescription Scutellaria, Houttuynia, Citrus aurantium, Lithospermum, licorice five herbs, four peaks can be clear chemical composition. Conclusion: The established HPLC-DAD fingerprinting expresses the overall characteristics of the measurable components of the decoction of Yin Yin Jie Du Bao and improve the specificity of TCM quality control. The research mode of retrograde backtransformation extends the idea of mass study of large compound prescription.