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1983—1984年在南京本校大豆研究室试验田内的“广吉”品种上分离到K_1和K_2两个病毒分离物。根据它们的寄主范围、体外抗性和蚜虫介体传病特性,电镜观察病毒粒体形态和内含体特征,以及SDS——琼脂双扩散的血清学反应,证实了K_1和K_2两个分离物都是属于马铃薯Y病毒组的大豆花叶病毒(SMV)。在大豆花叶病毒株系鉴别寄主上,K_1和K_2分离物与已鉴定的大豆花叶病毒S_A、S_B、S_C和S_E几个江苏株系的反应不同,主要是K_1和K_2两个株系都能系统侵染大豆“广吉”品种。K_1表现为系统轻斑驳花叶,而K_2为系统枯斑和叶脉坏死。此外,K_1不能侵染大豆品种“齐黄1号”和蚕豆品种“三白”等。以上证明,K_1和K_2两个分离物是江苏大豆花叶病毒(SMV)上的新株系。为了统一大豆花叶病毒的株系编号,将K_1和K_2分离物分别定名为S_G和S_H株系。
1983-1984 Two K_1 and K_2 virus isolates were isolated from “Guangji” cultivars in the soybean field of Nanjing Soybean Research Laboratory. Based on their host range, resistance in vitro and the disease-causing characteristics of aphids, electron microscopy was used to characterize the morphology and inclusion body of the virus and the serological reaction of SDS-agar double diffusion. The results showed that two isolates of K_1 and K_2 All belong to the potato virus Y virus group mosaic virus (SMV). The K_1 and K_2 isolates had different responses to several identified Jiangsu soybean mosaic virus strains S_A, S_B, S_C and S_E, mainly K_1 and K_2, in the identification host of soybean mosaic virus Soybean system can be infected with “wide Kyrgyzstan” varieties. K_1 is characterized by light motley mosaic leaf system, while K 2 is systemic necrosis and necrosis. In addition, K_1 can not infect the soybean variety “Qihuang 1” and the broad bean variety “San Bai”. The above proof, K_1 and K_2 two isolates Jiangsu soybean mosaic virus (SMV) on the new strains. In order to unify the soybean mosaic virus line number, K_1 and K_2 isolates were designated as S_G and S_H lines, respectively.