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目的 研究人类乳头状瘤病毒 (HPV)与肺鳞癌发生的关系。方法 采用聚合酶链反应(PCR)技术检测石蜡包埋肺鳞癌组织标本 50份、肿瘤组织旁鳞状化生上皮标本 3 0份、正常支气管粘膜3 0份中的HPVDNA。结果 三种标本中 ,HPVDNA的检出率分别为 2 6%、3 6.7%和 1 0 % ,其中以HPV1 6型所占比例最高。鳞状细胞癌和鳞状化生上皮标本中 ,HPV1 6与HPV6/ 1 1的检出率无明显差异 (P >0 .0 5)。结论 结果表明HPV感染在肺鳞癌的发生中可能起了重要的作用。鳞状上皮化生似可视为癌前病变。PCR技术较体外杂交技术和DNA探针有更高的敏感性。
Objective To study the relationship between human papilloma virus (HPV) and the incidence of lung squamous cell carcinoma. Methods Polymerase chain reaction (PCR) was used to detect 50 specimens of paraffin-embedded squamous cell carcinoma, 30 specimens of squamous metaplastic epithelium adjacent to tumor tissue, and 30 HPV DNA in normal bronchial mucosa. Results Among the three types of samples, the detection rates of HPV DNA were 26%, 36.7% and 10%, respectively, of which HPV16 type was the highest. In squamous cell carcinoma and squamous metaplastic epithelial specimens, the detection rates of HPV16 and HPV6/11 were not significantly different (P > 0.05). Conclusions The results suggest that HPV infection may play an important role in the development of lung squamous cell carcinoma. Squamous metaplasia can be seen as precancerous lesions. PCR technology has higher sensitivity than in vitro hybridization technology and DNA probes.