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介绍￣（１８６）Ｒｅ（Ｓｎ）－ＨＥＤＰ的制备工艺，重点研究由ＳｅｐｈａｄｅｘＧ－７５和国产７１７型阴离子交换树脂组成的复合柱进行放化纯度分析的方法。用高压液相色谱对原料、纯化前后的￣（１８６）Ｒｅ（Ｓｎ）－ＨＥＤＰ作ＵＶ吸收和对应放射性峰比对分析，实验结果表明，ＨＰＬＣ色谱图与文献引用的￣（９９）Ｔｃ－ＨＥＤＰ、￣（１８６）Ｒｅ（Ｓｎ）－ＨＥＤＰ的ＨＰＬＣ色谱图基本一致，产品中杂质含量远比文献中的同类产品要少。原来采用的生理盐水和丙酮纸色层分析体系并不能进行放化纯度的分析测定，尤其是在还原不充分、标记率不高的情况下。而复合柱色层却能快速、简便地进行放化纯度的测定，并能对产品进行纯化。经动物实验证明，纯化的￣（１８６）Ｒｅ（Ｓｎ）－ＨＥＤＰ在动物体内分布较理想，小鼠的甲状腺、肝等器官的吸收比未经纯化的￣（１８６）Ｒｎ（Ｓｎ）－ＨＥＤＰ明显减少。
This paper introduces the preparation of Re (Sn) -HEDP, and focuses on the method of radiochemical purity analysis of composite column composed of Sephadex G-75 and domestic 717 anion exchange resin. The UV absorption and corresponding radioactive peaks of ~ (186) Re (Sn) -HEDP before and after purification were analyzed by high pressure liquid chromatography (HPLC). The experimental results showed that the HPLC chromatograms were similar to those of 99 Tc-HEDP , (186) Re (Sn) -HEDP HPLC chromatograms are basically the same, the product impurity content than the literature less than similar products. Originally used saline and acetone paper chromatography analysis system can not conduct radiochemical purity determination, especially in the insufficient reduction, labeling rate is not high. The composite column chromatography is fast, easy to conduct radiochemical purity determination, and the product can be purified. The animal experiments show that purified (186) Re (Sn) -HEDP is well distributed in animals, and the absorption of thyroid, liver and other organs in mice is obviously higher than that of un-purified (186) Rn cut back.