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研究了克雷伯氏杆菌转化甘油生产1,3-丙二醇的底物流加控制策略,其特征是批式流加发酵过程与细胞生长和代谢相耦联.通过细胞生长动力学分析,建立了对数生长期和稳定期底物消耗与生物量和碱液消耗量之间的函数关系.2次在线反馈补料发酵实验结果表明,甘油浓度控制在20±2g/L时,1,3-丙二醇的终浓度分别达80.3和78.8g/L以上,与手动反馈补料相比,1,3-丙二醇浓度分别提高了25.0和23.5g/L.
The substrate flow control strategy of producing 1, 3-propanediol from glycerol by Klebsiella was studied, which is characterized by batch-fed fermentation coupled with cell growth and metabolism.According to cell growth kinetic analysis, Number of growth and stability of substrate consumption and biomass and lye consumption as a function of the relationship between .2 online feed fed-batch fermentation results showed that glycerol concentration control at 20 ± 2g / L, 1,3-propanediol The final concentrations were above 80.3 and 78.8 g / L, respectively, and the concentrations of 1,3-propanediol were increased by 25.0 and 23.5 g / L, respectively, compared with those fed by manual feeding.