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为深入开展流行性出血热（ＥＨＦ）流行病学和病原学方面的工作，作者应用Ｖｅｒｏ－Ｅ６细胞培养技术，从７份褐家鼠肺中分离到１株流行性出血热病毒（ＥＨＦＶ），从１１份ＥＨＦ急性期病人血清中分离到５株ＥＨＦＶ，从３份急性期病人尿中分离到１株ＥＨＦＶ。以兔抗ＥＨＦＶ多价血清混合单克隆抗体（ＭｃＡｂ），野鼠型特异ＭｃＡｂＨ５，家鼠型特异ＭｃＡｂＲ３１。经间接免疫荧光技术（ＩＦＡＴ）检测均证实为ＥＨＦＶ。用１４株ＥＨＦＶＭｃＡｂ对其中３株病毒（Ｈｕ，Ｂ２，Ｂ６株）抗原进行了分析，结果表明这３株病毒除具有ＥＨＦＶ组特异性抗原决定簇，还具有野鼠型特异性抗原决定簇，与野鼠型Ａ９，ＨＮ株反应模式基本一致，属野鼠型ＥＨＦＶ。
To further develop the epidemiology and etiology of epidemic hemorrhagic fever (EHF), an epidemic hemorrhagic fever virus (EHFV) was isolated from 7 Rattus norvegicus lungs using Vero-E6 cell culture technique. Five EHFV isolates were isolated from the serum of 11 patients with acute EHF, and one EHFV was isolated from the urine of 3 acute patients. Rabbit anti-EHFV polyvalent serum mixed monoclonal antibody (McAb), wild-type mouse McAbH5, home mouse-specific McAbR31. Indirect immunofluorescence (IFAT) test confirmed EHFV. Antigens of three strains of viruses (Hu, B2 and B6) were analyzed using 14 strains of EHFVMcAb. The results showed that these three strains of viruses except eHFV group had specific antigenic determinants, Type A9, HN strain basically the same reaction mode, is a wild rat type EHFV.