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苏木Caesalpinia sappan L.(10kg)用甲醇(50L)提取,减压蒸发,残渣溶于水,用乙酸乙酯提取3次。提取物经硅胶层析,以氯仿 (5L),氯仿-甲醇(95:5,90:10,85:15和80:20)洗脱,每次5L。取氯仿-甲醇部分的5％进行蒸发,再经Sephadex LH-20柱层析,用甲醇洗脱,得A、B、C三部分。蒸发C部分,从乙醇-水中结晶,得到490mg(I)的无色晶体,为苏木苦素P(caesalpin P)。其(100μg/ml)对兔血小板聚集的抑制率为54％。从苏木中还得到三个新的活性成分Ⅱ～Ⅳ,服用这些化合物皆安全,如Ⅲ,小鼠口服LD_(50)大于2g/kg。
Sumitomo Caesalpinia sappan L. (10 kg) was extracted with methanol (50 L) and evaporated under reduced pressure. The residue was dissolved in water and extracted three times with ethyl acetate. The extract was chromatographed on silica gel eluting with chloroform (5 L), chloroform-methanol (95:5, 90:10, 85:15, and 80:20) at 5 L each time. Take 5% of the chloroform-methanol fraction to evaporate, and then column chromatography on Sephadex LH-20 elute with methanol to obtain A, B, and C fractions. Part C was evaporated and crystallized from ethanol-water to give 490 mg of colorless crystals of (I) as caesalpin P. The inhibition rate of rabbit platelet aggregation (100 μg/ml) was 54%. Three new active ingredients II-IV were also obtained from the hematoxylin, and it was safe to take these compounds, such as III, mice oral LD 50 greater than 2g/kg.