用反相高效液相色谱法分离和鉴定九种抗生素类药物

• 作 者: 赵体慧
• 出 处: 南药译丛
• 发表日期: 1986年01期
• 关键词: 抗生素类药物 抗生素制剂 不锈钢柱 保留时间 填装 样品液 能将 流动相 蔡青 进样量

介绍用简便快速的反相高效液相色谱分离与鉴定羟氨苄青霉素、氨苄青霉素、邻氰青霉素、双氯青霉素、甲氧苯青霉素、苯唑青霉素、乙氧萘青霉素、青霉素 G 钾盐及青霉素 V 钾盐混合物的方法。一次进样能将九种抗生素分离,分析一次在30分钟内完成。此法灵敏,具有重现性,并可应用于抗生素制剂的定性分析。具体方法与条件:色谱柱:30cm×4.6mm id 不锈钢柱,用10μm Chromegabond C_(18)填装;柱温:室温;

INTRODUCTION The separation and identification of amoxicillin, ampicillin, meclofenox, dicloxacillin, methicillin, oxacillin, phenacillin, penicillin G and penicillin V by simple and rapid reversed-phase high performance liquid chromatography Potassium salt mixture. Nine injections of one antibiotic can be separated and analyzed in less than 30 minutes. This method is sensitive and reproducible and can be applied to the qualitative analysis of antibiotic preparations. Specific methods and conditions: Column: 30cm × 4.6mm id stainless steel column packed with 10μm Chromegabond C_ (18); column temperature: room temperature;