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目的:探讨大承气颗粒药物血清对小鼠肠上皮内淋巴细胞(intestinal intraepithelial lymphocytes,IELs)产生IL-2和IL-6的作用。方法:制备IELs,单次灌胃多次采血和以大黄酚t1/2(e)为间隔多次灌胃一次采血无菌制备药物血清,体外作用于IELs,用放免法检测IL-2和IL-6。结果:不同浓度的大承气颗粒剂用药后多数时间点的药物血清和多次给药的药物血清组IL-2显著高于培养液对照和IELs对照组;6.025g/kg大承气颗粒剂用药后9h,24h的药物血清的作用强于正常大鼠血清;用药后1.5h,1.205g/kg和6.025g/kg大承气颗粒剂的作用强于0.241g/kg大承气颗粒剂;多次给药的药物血清增加IL-2的作用强于正常血清,血清稀释后的作用显著减弱。单用大黄素、大黄酚和厚朴酚不能增加IL-2的产生。大承气颗粒药物血清引起IELs产生IL-2的量是IL-6的100余倍。结论:大承气颗粒药物血清具有增强小鼠肠上皮内淋巴细胞产生IL-2及少量IL-6的作用,以维护肠道免疫屏障,为其防治SIRS/MODS的可能机理之一。
Objective: To investigate the effect of Dachengqi Granule drug serum on the production of IL-2 and IL-6 in mouse intestinal intraepithelial lymphocytes (IELs). METHODS: IELs were prepared by multiple gavage with single gavage and drug sera were prepared by repeated gavage with chrysanthemum t1/2(e) at intervals. In vitro IELs were tested and IL-2 and IL were detected by radioimmunoassay. -6. RESULTS: IL-2 was significantly higher in drug serum and drug serum after multiple doses of Dachengqi granules with different concentrations than those in the control group and IELs control group; 6.025g/kg Dachengqi granules The effect of drug serum at 9h and 24h after drug administration was stronger than that of normal rats. At 1.5 h after administration, the effects of 1.125 g/kg and 6.025 g/kg Dachengqi granules were stronger than 0.241 g/kg Dachengqi granules. The multiple administration of drug serum increased the effect of IL-2 stronger than normal serum, and the effect of serum dilution was significantly reduced. The use of emodin, chrysophanol and honokiol alone did not increase IL-2 production. Dachengqi Granule drug serum caused IELs to produce more than 100 times more IL-2 than IL-6. Conclusion: Dachengqi Granule drug serum can enhance the production of IL-2 and a small amount of IL-6 in mouse intestinal epithelial lymphocytes to maintain the intestinal immune barrier and be one of the possible mechanisms for the prevention and treatment of SIRS/MODS.