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提出了苏云金芽胞杆菌毒蛋白与阿维菌素化学耦合的方法 ,实现了多位点生物杀虫毒素 Bt A的工业化生产。认为 Bt A的化学耦合 ,一方面是将 Bt的伴胞晶体进行酶切改造 ,形成带末端氨基的原毒素 ,另一方面是将放线菌的杀虫毒素阿维菌素通过对 C2 3 位置上的羟基进行激活、衍生化 ,形成带羧基的阿维菌素衍生物 ,最后利用氨基 -羧基耦联剂 EDC进行耦合 ,实现单体双生物毒素 Bt A的结构改造。高效生物杀虫剂 Bt A的研究成功 ,使其杀虫谱扩大为五个目 (鳞翅目、同翅目、双翅目、鞘翅目和螨类 ) ,杀虫速率提高为 >85 %死亡率 /2 4小时 ,保持了无公害的特性 ,对于农业环保具有重要意义
The method of chemical coupling between the thuringiensis toxin protein and abamectin was proposed, and the industrial production of multi-site biocidal toxin Bt A was realized. Bt A chemical coupling is considered, on the one hand is Bt companion crystal digestion transformation to form a protoxin with a terminal amino group, the other is the actinomycin insecticidal toxin avermectin C2 3 position On the hydroxyl activation, derivatization, the formation of carboxyl-containing avermectin derivatives, and finally the use of amino-carboxyl coupling agent EDC coupling to achieve the structure of the monomer biotoxin Bt A structural transformation. The success of the highly effective biocide Bt A has resulted in the expansion of its insecticidal spectrum to five orders (Lepidoptera, Homoptera, Diptera, Coleoptera and Mites) with an insecticidal rate of> 85% mortality Rate / 2 4 hours, to maintain the characteristics of pollution-free, for agricultural environmental protection is of great significance