【摘 要】
：
利用3种诱变方法对黑曲霉3.316进行诱变,得到1株β-葡萄糖苷酶活力最高的菌株(编号为Ch2),并对该菌株进行产酶条件优化试验.结果表明:诱变菌株Ch2的最佳产酶条件为氮源为蛋白
【机 构】
：
湖北襄樊学院化学工程与食品科学学院
论文部分内容阅读
利用3种诱变方法对黑曲霉3.316进行诱变,得到1株β-葡萄糖苷酶活力最高的菌株(编号为Ch2),并对该菌株进行产酶条件优化试验.结果表明:诱变菌株Ch2的最佳产酶条件为氮源为蛋白胨,质量浓度2％,碳源为麸皮,质量浓度2％,表面活性剂吐温- 80加入量2 mL,培养温度35℃,通气量200 r/min,接种量为1.5％,在此条件下培养72 h,诱变菌株Ch2的β-葡萄糖苷酶活力为302.96 U/mL,比优化前的酶活力提高了6.4％,比诱变前菌株的酶活力提高了86.5％.
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