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目的:优选湿毒清胶囊中地黄的提取工艺并比较地黄切制前后的药效。方法:采用HPLC测定梓醇含量,流动相乙腈-0.1%磷酸溶液(1∶99),检测波长210 nm。以梓醇提取量为指标,采用正交试验考察粉碎度、料液比、提取时间对地黄提取工艺的影响。以生理盐水为空白对照,地塞米松为阳性对照,通过抗炎镇痛试验比较地黄药材切制前后高(16 g·kg-1)、低剂量组(8 g·kg-1)的药效差异。结果:最佳提取工艺为粉碎成5 mm小块,加20倍量水提取2次,每次1.5 h。切制前后高剂量组对二甲苯诱发小鼠耳廓肿胀的抑制率分别为33.72%,48.34%;低剂量组依次为43.13%,64.31%。结论:该工艺稳定可行,为湿毒清胶囊的工业生产提供参考。
Objective: To optimize the extraction process of Radix Rehmanniae Capsules and compare the efficacy before and after Radix Rehmanniae Preparata. Methods: The catalpol content was determined by HPLC. The mobile phase consisted of acetonitrile - 0.1% phosphoric acid solution (1:99) and the detection wavelength was 210 nm. Taking catalpol extraction as index, orthogonal experiment was conducted to investigate the influence of pulverization, solid-liquid ratio and extraction time on extraction technology of Rehmannia glutinosa. Physiological saline as a blank control, dexamethasone as a positive control, anti-inflammatory analgesic effect of Rehmanniae Radix rehmanniae Preparata (16 g · kg-1) and low dose group (8 g · kg-1) difference. Results: The best extraction process was pulverized into 5 mm pieces, plus 20 times the amount of water extracted twice, each 1.5 h. The inhibitory rates of xylenyl-induced auricle swelling in high-dose and high-dose groups were 33.72% and 48.34%, respectively. The low dose group was 43.13% and 64.31%, respectively. Conclusion: The process is stable and feasible, providing a reference for the industrial production of wet drug capsules.