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This paper developes a temperature control system in order to carry out a real time and continuous observation on living cells under the microscope.In this system,transparent indium tin oxid(ITO) conductive film was used as a heater,temperature was measured by the temperature dependence of resistance behavior of the ITO film,C8051F340 single-clup was used as the control hardware core,and the modified PID algorithm and pulse width modulation(PWM) were adopted as the key control software programs.The temperature system had a simple structure without added temperature sensor,an uniform temperature distribution within ±1℃ on the ITO film surface and temperature control precision of ±0.2℃ could be obtained.The system could maintain a good growth state for BHK-21 living cell under the microscope for 48 h.
This paper developes a temperature control system in order to carry out a real time and continuous observation on living cells under the microscope. This system, transparent indium tin oxid (ITO) conductive film was used as a heater, temperature was measured by the temperature dependence of resistance behavior of the ITO film, C8051F340 single-clup was used as the control hardware core, and the modified PID algorithm and pulse width modulation (PWM) were adopted as the key control software programs. The temperature system had a simple structure without added temperature sensor, an uniform temperature distribution within ± 1 ° C on the ITO film surface and temperature control precision of ± 0.2 ° C could be obtained. The system could maintain a good growth state for BHK-21 living cell under the microscope for 48 h.