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目的研究别嘌呤醇对高果糖诱导的胰岛素抵抗性的影响。方法 Wistar雄性大鼠随机分为正常组、模型组和实验组。实验组和模型组,以高果糖饲料喂养同时用别嘌呤醇(25μg·g~(-1))或等量双蒸水灌胃4周。用口服葡萄糖耐量实验法和高胰岛素-正常葡萄糖钳夹法,研究别嘌呤醇对胰岛素敏感性的作用,测定各组空腹血糖、空腹胰岛素水平和血清尿酸水平的变化。结果血清尿酸水平,与正常组的(1.33±0.12)mg·d L~(-1)相比,模型组(1.82±0.10)mg·d L~(-1)明显增高(P<0.05);与模型组相比,实验组(0.90±0.27)mg·d L~(-1)能明显改善由高果糖引起的尿酸浓度的增高(P<0.05)。不同时间段各组之间血糖值无明显差异。模型组的胰岛素敏感性指标(GIR)明显低于正常组(P<0.05),表明出现胰岛素抵抗性;实验组的GIR值明显高于模型组(P<0.05)。结论别嘌呤醇可以提高骨骼肌胰岛素敏感性,明显改善高果糖诱导的胰岛素抵抗性。
Objective To study the effect of allopurinol on insulin resistance induced by high fructose. Methods Wistar male rats were randomly divided into normal group, model group and experimental group. The experimental group and the model group were fed with high fructose diet and gavaged with allopurinol (25μg · g -1) or the same amount of double distilled water for 4 weeks. The effects of allopurinol on insulin sensitivity were studied by oral glucose tolerance test and hyperinsulinemic-normal glucose clamp, and the changes of fasting blood glucose, fasting insulin and serum uric acid in each group were determined. Results Compared with normal group (1.33 ± 0.12) mg · d L -1, the level of serum uric acid in model group was significantly higher (1.82 ± 0.10) mg · d L -1 (P <0.05). Compared with the model group, the experimental group (0.90 ± 0.27) mg · d L -1 could obviously improve the increase of uric acid concentration caused by high fructose (P <0.05). There was no significant difference in blood glucose between groups at different time points. The insulin sensitivity index (GIR) of the model group was significantly lower than that of the normal group (P <0.05), indicating insulin resistance. The GIR of the experimental group was significantly higher than that of the model group (P <0.05). Conclusion Allopurinol can improve insulin sensitivity of skeletal muscle and significantly improve insulin-induced insulin resistance.