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研究背景 自70年代初期国外建立血清铁蛋白实验检测方法以来,把铁缺乏症分为非贫血型和贫血型。为进一步深入研究,应首先确定两型血红蛋白分界指标,即明确血红蛋白正常值低限。 研究方法 实验组共152例,年龄6～7岁,青海省西宁市29例,贵德县24例,共和县26例,贵南县40例,玛多县33例;对照组由北京市36名6～7岁健康儿童组成。用高铁氰化法检测血红蛋白,目镜目测计数红细胞,并测定压积红细胞、压积红细胞重量、游离原叶啉。 研究结果 有关血红蛋白三项指标(血红蛋白、平均红细胞血红蛋白、平均红细胞血红蛋白浓度);有关红细胞三项指标(红细胞、压积红细胞、平均红细胞容积);红细胞重量两项指标(压积红细胞重量、平均红细胞重量);红细胞游离原叶啉两项指标(游离原卟啉、平均红细胞原卟啉)实验组与对照组相比较均有极显著差异。 结论 不同地区儿童,以上10项指标的检测值各不相同,且随海拔高度的增加各项指标均有不同程度增加。建立这10项指标的正常值对我国铁缺乏症和高原乏氧的研究具有十分重要的意义。
Background of the study Since the early 1970s, the establishment of foreign ferritin test method since the iron deficiency is divided into non-anemia and anemia. For further study, we should first determine the two types of hemoglobin demarcation index, that is, the lower limit of normal hemoglobin. METHODS: The experimental group consisted of 152 patients aged 6-7 years, 29 cases of Xining City in Qinghai Province, 24 cases of Guide County, 26 cases of Gonghe County, 40 cases of Guinan County and 33 cases of Maduo County. The control group consisted of Beijing 36 Name 6 to 7-year-old healthy children. Hemoglobin was detected by high-speed ferricyanide method, and erythrocytes were counted visually by eyepieces. The weight of red blood cells and the weight of erythrocytes were measured. The results of the three indicators of hemoglobin (hemoglobin, mean erythrocyte hemoglobin, mean erythrocyte hemoglobin concentration); three indicators of red blood cells (red blood cells, hematocrit, mean corpuscular volume); two indicators of red blood cell weight (red blood cell weight, Weight); two indicators of red cell free protoporphyrin (free protoporphyrin, mean erythrocyte protoporphyrin) experimental group compared with the control group were significantly different. Conclusion Different regions of children, the above 10 indicators of the measured values vary, and with the altitude increased the indicators have increased in varying degrees. The establishment of these 10 indicators of normal values of iron deficiency deficiency and altitude hypoxia research is of great significance.