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目的建立用于分子药理学研究的药物分离吸附层析模拟系统并初步应用。方法提出吸附层析的多室解吸理论模型 ,推导相关公式 ,编制计算机程序。结果与结论该模型可根据实验需要拟合出最佳分离效果的吸附层析解吸曲线 ,并准确描述系统各室浓度随时间的变化规律。介绍了数例有应用价值的特殊解吸曲线 ,并利用此系统对膜整合蛋白进行了分离 ,取得了较传统两室线性解吸法明显好的分离效果
OBJECTIVE To establish a drug separation and adsorption chromatography simulation system for molecular pharmacology research and preliminary application. Methods The multi-chamber desorption theoretical model of adsorption chromatography was proposed, the relevant formulas were deduced, and the computer program was compiled. Results and Conclusion According to the experimental results, the model can fit the best chromatographic desorption curve of adsorption chromatography and accurately describe the variation of concentration in each chamber with time. Several special desorption curves with application value were introduced and the membrane integral protein was separated by this system. The separation effect was obviously better than the traditional two-chamber linear desorption method