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目的:探讨microRNA-125b(miR-125b)在人视网膜母细胞瘤细胞中的表达,及其对肿瘤细胞增殖抑制和诱导凋亡的作用。方法:采用RT-PCR方法检测miR-125b在视网膜母细胞瘤细胞系Y79中的表达;构建miR-125b过表达(miR-125bmimic)和抑制载体(miR-125inhibitor)转染Y79细胞,检测miR-125b表达情况;通过细胞凋亡实验和细胞增殖实验(CCK-8)观察miR-125b过表达和抑制表达后,对肿瘤细胞增殖力和凋亡的影响。结果:miR-125b在人视网膜母细胞瘤细胞系中Y79细胞系中呈高表达;miR-125bmimic组(Y79+miR-125bmimic)中miR-125b的表达较miR-NC组(未处理的Y79细胞)表达显著增多,差异具有统计学意义(P<0.05);miR-125binhibitor组(Y79+miR-125binhibitor)中miR-125b的表达较miR-NC组表达显著减少,差异具有统计学意义(P<0.05);miR-125bmimic组较miR-NC组相比,Y79细胞增殖力显著增高,细胞凋亡显著下降,差异具有统计学意义(P<0.05);而miR-125inhibitor组较miR-NC组相比肿瘤细胞增殖力显著下降,细胞凋亡率显著提高,差异具有统计学意义(P<0.05)。miR-125b可能是通过DRAM2基因来发挥其致癌作用;结论:miR-125b可能作为癌基因在视网膜母细胞瘤中发挥作用,并对肿瘤细胞增殖和凋亡具有显著影响。
Objective: To investigate the expression of microRNA-125b (miR-125b) in human retinoblastoma cells and its effect on tumor cell proliferation inhibition and apoptosis induction. Methods: The expression of miR-125b in retinoblastoma cell line Y79 was detected by RT-PCR. Y79 cells were transfected with miR-125bmimic and miR-125inhibitor to detect the expression of miR- 125b expression. The effect of miR-125b overexpression and inhibition on the proliferation and apoptosis of tumor cells was observed by cell apoptosis assay and cell proliferation assay (CCK-8). Results: miR-125b was highly expressed in Y79 cell line of human retinoblastoma cell line; miR-125b expression in miR-125bmimic group (Y79 + miR-125bmimic) was significantly higher than that in miR-NC group (P <0.05). The expression of miR-125b in miR-125binhibitor group was significantly lower than that in miR-NC group (P <0.05), and the difference was statistically significant (P < 0.05) .Compared with miR-125 group, the proliferation of miR-125bmimic group was significantly increased, while the apoptosis of miR-125bmimic group was significantly lower than that of miR-125bmimic group (P <0.05) Compared with the proliferation of tumor cells, the apoptosis rate was significantly decreased, the difference was statistically significant (P <0.05). miR-125b may exert its oncogenic effect through the DRAM2 gene; Conclusion: miR-125b may play an important role as an oncogene in retinoblastoma and have a significant effect on tumor cell proliferation and apoptosis.