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目的探讨褪黑素(MT)对脑损伤的保护作用。方法 72只妊娠第19天SD孕鼠随机均分为三组:A组,腹腔注射细菌脂多糖(LPS)500μg/kg;B组,腹腔注射LPS 500μg/kg+MT10 mg/kg;C组,腹腔注射等容积生理盐水。分别于给药后1、6、12、24、48、72 h剖腹取出胎鼠,急性分离脑组织,新型荧光探针Fluo-3/AM负载胎鼠脑细胞,流式细胞术检测胎鼠细胞内游离钙的平均荧光强度。结果注药后,A、B组脑细胞钙离子荧光值于6 h开始明显上升,12 h达高峰,各观测时点的钙离子荧光值均明显高于C组(P<0.01)。除72 h外,B组其他各观测时点的钙离子荧光值均明显低于A组(P<0.01或P<0.05)。结论 LPS可诱导胎鼠脑细胞内钙离子超载,导致脑损伤。MT通过抑制细胞内钙超载对脑细胞损伤有保护作用。
Objective To investigate the protective effect of melatonin (MT) on brain injury. Methods 72 pregnancies on day 19 of gestation were randomly divided into three groups: group A, intraperitoneal injection of bacterial lipopolysaccharide (LPS) 500μg / kg; group B, intraperitoneal injection of LPS 500μg / kg + MT 10 mg / kg; group C, Intraperitoneal injection of equal volume of saline. Fetal rats were removed by intraperitoneal injection at 1, 6, 12, 24, 48 and 72 h after administration respectively. Acutely isolated brain tissues were cultured with Fluo-3 / AM, a new fluorogenic probe. Brain cells were detected by flow cytometry Intracellular free calcium average fluorescence intensity. Results After injection, calcium fluorescence of brain cells in groups A and B began to increase significantly at 6 h and peaked at 12 h. The calcium fluorescence of each group was significantly higher than that of group C (P <0.01). Except for 72 h, the calcium fluorescence of other groups in group B was significantly lower than that in group A (P <0.01 or P <0.05). Conclusion LPS can induce calcium overload in fetal rat brain cells and lead to brain injury. MT has a protective effect on brain cell injury by inhibiting intracellular calcium overload.