论文部分内容阅读
目的:研究胰岛素分泌细胞的体外诱导方法及其对大鼠糖尿病的疗效。方法:分离培养大鼠骨髓干细胞,用尼克酰胺及肠促胰岛素类似物诱导其分化为胰岛素分泌细胞。将24只Wistar大鼠随机分为对照组、糖尿病组和诱导组。后两组建立糖尿病模型,将该胰岛素分泌细胞回输至诱导组体内,监测大鼠体重、血糖(空腹及OGTT 120分血糖)及空腹胰岛素等。实验终止时取大鼠肝脏、脾脏、胰腺,检测胰岛素和BrdU染色双阳性细胞。结果:治疗后诱导组大鼠胰岛素水平显著高于糖尿病组(P<0.05),空腹及OGTT 120分血糖均低于糖尿病组(P均<0.05)。诱导组胰腺中胰岛素阳性细胞百分比明显高于糖尿病组(P<0.05),诱导组的胰腺、肝脏及脾脏中发现少量胰岛素和BrdU双阳性细胞。结论:尼克酰胺联合肠促胰岛素类似物可诱导骨髓干细胞分化为胰岛素分泌细胞;该胰岛素分泌细胞能提高胰岛素水平,降低血糖,对大鼠糖尿病有明显治疗作用。
Objective: To study the in vitro induction of insulin-secreting cells and its therapeutic effect on diabetic rats. METHODS: Rat bone marrow stem cells were isolated and cultured, and induced to differentiate into insulin-secreting cells with nicotinamide and incretins. 24 Wistar rats were randomly divided into control group, diabetes group and induction group. The latter two groups were established diabetic model, the insulin-secreting cells were returned to the induction group body weight, blood glucose (fasting and OGTT 120 points of blood sugar) and fasting insulin and so on. At the end of the experiment, rat liver, spleen and pancreas were taken for detection of insulin and BrdU-stained double positive cells. Results: After treatment, the insulin level in the induced group was significantly higher than that in the diabetic group (P <0.05). The fasting blood glucose and blood sugar in 120 minutes of OGTT were lower than those in the diabetic group (all P <0.05). The percentage of insulin positive cells in the pancreas of induction group was significantly higher than that of diabetic group (P <0.05). A small amount of insulin and BrdU double positive cells were found in the pancreas, liver and spleen of the induced group. CONCLUSION: Nicotamide combined with incretin analogs can induce bone marrow stem cells to differentiate into insulin-secreting cells. The insulin-secreting cells can increase insulin level, lower blood glucose and have obvious therapeutic effect on diabetic rats.