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作者用放免法在113例乳腺癌和354例女性供血者中检测血清脑型肌酸激酶(CPK-BB)含量.在放免检测时,与酶的MB型(不是MM型)有部份交义反应,但MB型有与稀释度不相平行的特点,据此可与酶的BB型区别.检测中批内变异为4.6%,而批间变异为10.3%.标本在采集后12小时内分离,此后或立即检测或贮存于-70℃达一周以上.对高含量患者的血清作一系列稀释后制成曲线,并与已检测的标准曲线比较.
The authors used serum radioimmunoassay to detect serum brain creatine kinase (CPK-BB) levels in 113 cases of breast cancer and 354 female blood donors. When tested for radioimmunoassay, they were partially synonymous with the enzyme MB type (not MM type). The reaction, but the MB type is not parallel to the dilution, according to which can be distinguished with the BB type of enzyme. The intra-assay variation is 4.6% and the inter-assay variation is 10.3%. The specimen is separated within 12 hours after collection. , Afterwards or immediately detected or stored at -70 °C for more than one week. After a series of dilutions of serum in high-content patients made curve, and compared with the standard curve has been detected.