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目的探讨霉酚酸酯(MMF)在糖尿病肾组织中发挥抗炎作用及其机制。方法MMF治疗2型糖尿病大鼠,观察肾组织单核/巨噬细胞浸润,及转录因子(NF)-κB p65、神经生长因子表达的变化;不同浓度的霉酚酸干预高糖环境下培养的肾小球系膜细胞,测定细胞间粘附分子(ICAM)-1、单核细胞趋化蛋白(MCP)-1及神经生长因子(NGF)表达的变化。结果MMF治疗能显著改善糖尿病大鼠肾功能,伴肾小球NF-κB p65活性明显下降,NGF表达水平明显降低,单核/巨噬细胞浸润显著减轻。霉酚酸对高糖诱导的肾小球系膜细胞ICAM-1、MCP-1和NGF高表达有显著的抑制作用。结论MMF很可能通过降低NF-κB活性,间接抑制炎性因子表达、炎症细胞浸润。霉酚酸对高糖环境下肾小球系膜细胞ICAM-1、MCP-1和NGF的高表达有显著抑制作用。
Objective To investigate the anti-inflammatory effect and mechanism of mycophenolate mofetil (MMF) in diabetic renal tissue. Methods MMF was used to treat rats with type 2 diabetes mellitus (DM) to observe the infiltration of monocytes / macrophages and the expression of NF-κB p65 and NGF in renal tissues. The effects of different concentrations of mycophenolic acid Glomerular mesangial cells were cultured and the changes of ICAM-1, MCP-1 and NGF were measured. Results The treatment of MMF could significantly improve the renal function of diabetic rats. The activity of NF-κB p65 in glomeruli was significantly decreased, the expression of NGF was significantly decreased and the infiltration of monocytes / macrophages was significantly reduced. Mycophenolate mofetil had a significant inhibitory effect on the high expression of ICAM-1, MCP-1 and NGF in mesangial cells induced by high glucose. Conclusion MMF may indirectly inhibit the expression of inflammatory cytokines and infiltration of inflammatory cells by decreasing NF-κB activity. Mycophenolate mofetil had a significant inhibitory effect on the high expression of ICAM-1, MCP-1 and NGF in glomerular mesangial cells under high glucose condition.